PD-L1 Antibody / B7-H1 / CD274

• Catalog Number: orb1822739
• Category: Antibodies
• Description: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 190-290) from the human protein was used as the immunogen for the B7-H1 antibody.
• UniProt ID: Q9NZQ7
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: PDL1/8809R
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Aliquot the B7-H1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This B7-H1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human placental tissue with B7-H1 antibody (clone PDL1/8809R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free B7-H1 antibody (clone PDL1/8809R) as confirmation of integrity and purity.