PD-1 Antibody

• Catalog Number: orb1906340
• Category: Antibodies
• Description: PD-1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1G4
• Tested applications: ELISA, FC
• Reactivity: Human, Primate
• Isotype: IgG
• Immunogen: The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human PD-1 protein.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: PD-1
• UniProt ID: Q15116
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD279; PD1; PD 1; hPD-1; PDCD1; Programmed cell de
• Note: For research use only
• Application notes: The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey PD-1 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey PD-1 and not mouse PD-1. The binding affinity of the original mouse IgG4 antibody for human PD-1 was determined using surface plasmon resonance and was found to be Kd= 1.07 nM. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hPD-1 mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257).
• Expiration Date: 12 months from date of receipt.