PDCD1LG2 Antibody

• Catalog Number: orb1239861
• Category: Antibodies
• Description: PDCD1LG2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-PD-L2 antibody (orb1239861) was raised against a peptide corresponding to 16 amino acids near the center of human PD-L2. The immunogen is located within amino acids 140-190 of PD-L2.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 31kDObserved: 29 kD
• Target: PDCD1LG2
• UniProt ID: Q9BQ51
• NCBI: NP_079515
• Storage: PD-L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Raji Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: PD-L2 orb1239861 (A: 0.5 µg/mL and B: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PD-L2, orb1239861 (4 µg/mL), competitor antibody (4 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

KO Validation in HeLa Cells. Loading: 15 µg of HeLa WT cell lysates or PD-L2 KO cell lysates. Antibodies: PD-L2, orb1239861 (4 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

KO Validation in HeLa Cells. Loading: 15 µg of HeLa WT cell lysates or PD-L2 KO cell lysates. Antibodies: PD-L2, orb1239861 (4 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of PD-L2 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PD-L2 antibody (orb1239861) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of PD-L2 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling PD-L2 with orb1239861 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of PD-L2 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PD-L2 antibody (orb1239861) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of PD-L2 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling PD-L2 with orb1239861 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of PD-L2 in Lung Tumor of Mice (Kao et al., 2015). Protein analysis for PD-L2 (E-H) by immunohistochemistry with anti-PD-L2 antibodies in mice lung tumors. hMUC1.Tg mice were induced with lung adenoma and then treated with concurrent or sequential cisplatin/radiotherapy. PD-L2 expression level at week 41 after treatment was similar in control and treatment groups.

Regulated Expression Validation of PD-L2 in Mice with Melanoma Tumor (Knox et al., 2019). Immunoblot analysis of PD-L2 expression with anti-PD-L2 (orb1239861) antibodies. PD-L2 expression was up-regulated by anti-PD1 antibody treatment whereas it was reduced by Next A alone or combination treatment (anti-PD1 antibody + NextA).