PCSK9 Antibody

• Catalog Number: orb1249208
• Category: Antibodies
• Description: PCSK9 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: TRFHRQASKCDSHGT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human PCSK9 (myc and DYKDDDDK tagged), a doublet of approx. 80-100kDa was observed. No bands were observed in mock-transfected HEK293 and the same bands were observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PCSK9
• UniProt ID: Q8NBP7
• NCBI: NP_777596.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PCSK9, proprotein convertase subtilisin/kexin type
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human PCSK9 (myc and DYKDDDDK tagged), a doublet of approx. 80-100kDa was observed. No bands were observed in mock-transfected HEK293 and the same bands were observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Expiration Date: 12 months from date of receipt.

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PCSK9 with C-terminal MYC tag probed with orb1249208 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb1249208 (1 mg/ml) in Lane B.