PCGF1 Antibody

• Catalog Number: orb1257082
• Category: Antibodies
• Description: PCGF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-259 of human PCGF1 (NP_116062.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 30kDa
• Target: PCGF1
• UniProt ID: Q9BSM1
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: 2010002K04Rik, NSPC1, RNF3A-2, RNF68, polycomb gro
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PCGF1 antibody (orb1257082). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of L-929 cells using PCGF1 antibody (orb1257082) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using PCGF1 antibody (orb1257082) at dilution of 1:100. Blue: DAPI for nuclear staining.