PCDH20 Rabbit Polyclonal Antibody

• Catalog Number: orb1009
• Category: Antibodies
• Description: PCDH20 Rabbit Polyclonal Antibody
• Target: PCDH20
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human PCDH20 (611-710/924aa)
• UniProt ID: Q8N6Y1
• MW: 98 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PCD20_HUMAN; PCDH 13; PCDH 20; PCDH13; PCDH20; Pro
• Note: For research use only

Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-PCDH20 antibody (orb1009), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SV2B) Polyclonal Antibody, Unconjugated (orb1009) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hippocampus (Mouse) Lysate at 40 ug, Olfactory bulb (Mouse) Lysate at 40 ug, Primary: Anti-PCDH20 (orb1009) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 100 kD.

Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human U251 cell lysates, Lane 3: Human U87MG cell lysates, Primary: Anti-PCDH20 (orb1009) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kDa, Observed band size: 102 kDa.