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PCDH12 Rabbit Polyclonal Antibody

SKU: orb3025

Description

PCDH12 Rabbit Polyclonal Antibody

Research Area

Cadherins, Cell Biology, Neuroscience, Placental Development

Images & Validation

Tested ApplicationsFC, WB
Dilution RangeWB=1:500-2000, Flow-Cyt=1μg/Test
ReactivityMouse
Predicted ReactivityBovine, Equine, Human, Rat, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human PCDH12 (401-500/1184aa)
TargetPCDH12
Molecular Weight126 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PCD12_HUMAN; Pcdh12; Protocadherin 12; Protocadherin-12; Vascular cadherin 2; Vascular cadherin-2; Vascular endothelial cadherin 2; Vascular endothelial cadherin-2; VE cadherin 2; VE-cad-2; VE-cadherin-2; VECAD2.

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PCDH12 Rabbit Polyclonal Antibody

Positive control: (mo)Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody: Rabbit IgG, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody: Rabbit Anti-CD28 antibody (orb3025), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

PCDH12 Rabbit Polyclonal Antibody

Sample: SP2/0 (Mouse) Cell Lysate at 30 ug, Primary: Anti-PCDH12 (orb3025) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 126 kD, Observed band size: 126 kD.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol

PCDH12 Rabbit Polyclonal Antibody (orb3025)

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