PCDH12 Rabbit Polyclonal Antibody

• Catalog Number: orb3025
• Category: Antibodies
• Description: PCDH12 Rabbit Polyclonal Antibody
• Target: PCDH12
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Equine, Human, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human PCDH12 (401-500/1184aa)
• UniProt ID: Q9NPG4
• MW: 126 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PCD12_HUMAN; Pcdh12; Protocadherin 12; Protocadher
• Research Area: Cadherins, Cell Biology, Neuroscience, Placental D
• Note: For research use only

Positive control: (mo)Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody: Rabbit IgG, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody: Rabbit Anti-CD28 antibody (orb3025), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Sample: SP2/0 (Mouse) Cell Lysate at 30 ug, Primary: Anti-PCDH12 (orb3025) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 126 kD, Observed band size: 126 kD.