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Catalog Number | orb1523205 |
---|---|
Category | Antibodies |
Description | PCCA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Immunogen | Between 678 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | PCCA |
UniProt ID | P05165 |
NCBI | NP_000273.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | propionyl-CoA carboxylase alpha chain, mitochondri Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse PCCA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PCCA antibody orb1523205 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human PCCA by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-PCCA (Cat. No. orb1523205) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human PCCA by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PCCA (Cat. No. orb1523205) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human | |
Mouse | |
Polyclonal | |
Unconjugated |