CBX4 Antibody

SKU: orb345447

Description

PC2 antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Dilution Range: ELISA: 1:10,000 - 1:40,000, IF: 1:100 - 1:400, IP: 1:100, WB: 1:1,000 - 1:4,000
• Reactivity: Human
• Application Notes:
  Anti-PC2 antibody has been tested for use in ELISA, WB, and IF. This affinity purified antibody is useful in immunofluorescence staining of cultured cells. In immunofluorescence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. This affinity purified antibody is useful in western blotting using transfected cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein. Reactivity in other immunoassays is unknown.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 90-115 of Human PC2 protein.
• Target: CBX4
• Purity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PC2 protein.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.36 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PC2 antibody, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4

Analysis shows the detection of human PC2 in probed lysates using Biorbyt's Affinity Purified anti-hPC2 antibody.

Biorbyt's anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2. IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Triton X100-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37°C. Nuclei were counterstained with Hoechst stain (blue).

Quick Database Links

Gene Symbol

CBX4

UniProt

O00257

RefSeq:O00257.3