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Catalog Number | orb1528121 |
---|---|
Category | Antibodies |
Description | PBRM1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 1639 and 1689 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | PBRM1 |
UniProt ID | Q86U86 |
NCBI | AAP34197.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | BAF180; protein polybromo-1; polybromo-1D; PB1; BR Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human PBRM1 by western blot.Samples: Whole cell lysate from Jurkat, HeLa, and HEK293T cells. Antibody: Affinity purified rabbit anti-PBRM1 antibody orb1528121 at 0.1 µg/ml.
Detection of mouse PBRM1 by western blot.Samples: Whole cell lysate from NIH 3T3 and TCMK-1. Antibody: Affinity purified rabbit anti-PBRM1 antibody orb1528121 at 0.1 µg/ml.
Detection of human PBRM1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of mouse PBRM1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of human PBRM1 by western blot of immunoprecipitates.Samples: Whole cell lysate from Jurkat cells. Antibodies: Affinity purified rabbit anti-PBRM1 antibody.
FC, ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
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