PBRM1 Antibody

• Catalog Number: orb1519953
• Category: Antibodies
• Description: PBRM1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, SW-Size, WB
• Reactivity: Human, Mouse
• Immunogen: residues 1-293
• Concentration: 250 µg/ml
• Dilution range: WB - 1:1000; IP - 5 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PBRM1
• UniProt ID: Q86U86
• NCBI: AAP34197.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: BAF180; protein polybromo-1; polybromo-1D; PB1; BR
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PBRM1 in FFPE breast by immunohistochemistry-IF. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (orb1519953). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.

Detection of mouse PBRM1 by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (orb1519953) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.