PBK1 antibody

• Catalog Number: orb345591
• Category: Antibodies
• Description: PBK1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: 1:2,500 - 1:10,000, IF: 1:100, IP: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PBK1 protein. A BLAST analysis was used to suggest limited cross-reactivity with PBK1 from mouse based on a 75% homology with the immunizing sequence. Cross-reactivity with PBK1 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: O76021
• NCBI: 62901068
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PBK1 antibody, PBK-1, PBK 1, CATX-11,
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous PBK1. For immunofluorescence microscopy, fix cells with methanol.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of shown to co-localize with recombinant protein using PBK1 antibody

Western blot analysis of HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate using PBK1 antibody