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Catalog Number | orb234356 |
---|---|
Category | Antibodies |
Description | PBK Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human PBK recombinant protein (Position: N71-V322). Human PBK shares 89% amino acid (aa) sequence identity with mouse PBK. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 36085 MW |
UniProt ID | Q96KB5 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Lymphokine-activated killer T-cell-originated prot Read more... |
Note | For research use only |
Application notes | WB: The detection limit for PBK is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A549 cells using anti-PBK antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of PBK using anti-PBK antibody.Lane 1:human HeLa cell; 2:human HepG2 cell; 3:rat testis tissue; 4:mouse testis tissue.
IF analysis of PBK using anti-PBK antibody. PBK was detected in immunocytochemical section of U20S cells.
IHC analysis of PBK using anti-PBK antibody. PBK was detected in a paraffin-embedded section of mouse testis tissue.
IHC analysis of PBK using anti-PBK antibody. PBK was detected in a paraffin-embedded section of rat testis tissue.
IHC analysis of PBK using anti-PBK antibody. PBK was detected in a paraffin-embedded section of human mammary tissue.
IF, IH, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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