Pbk Antibody

• Catalog Number: orb1265516
• Category: Antibodies
• Description: Pbk Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This Mouse Pbk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of mouse Pbk.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: Pbk
• UniProt ID: Q9JJ78
• NCBI: Q9JJ78
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Lymphokine-activated killer T-cell-originated prot
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing A431 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H + L) (1583138) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysate from Hela cell line, using Mouse Pbk Antibody at 1:1000.

Western blot analysis of lysate from mouse testis tissue lysate, using Mouse Pbk Antibody at 1:1000 at each lane.