PAX8 Antibody

• Catalog Number: orb2640250
• Category: Antibodies
• Description: PAX-8 is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PAX8/1491
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A human recombinant fragment (aa 60-261) was used as the immunogen for the PAX-8 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PAX-8 antibody is available for research use only.
• UniProt ID: Q06710
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PAX-8 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of 1) human partial recombinant protein and 2) human Raji cell lysate with PAX-8 antibody (clone PAX8/1491). Predicted molecular weight of isoforms 1-5: 31, 35, 42, 43 and 48 kDa, respectively. PAX-8 can also be observed at ~62 kDa.