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PAX6 Rabbit Polyclonal Antibody

Catalog Number: orb612166

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb612166
CategoryAntibodies
DescriptionPAX6 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, WB
Predicted ReactivityBovine, Canine, Equine, Gallus, Rabbit, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human PAX6 (221-320/422aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW46 kDa
TargetPAX6
UniProt IDP26367
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesAN 2; AN antibody; AN2; Aniridia type II protein;
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NoteFor research use only
Expiration Date12 months from date of receipt.
PAX6 Rabbit Polyclonal Antibody

Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-PAX6 antibody (orb612166), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

PAX6 Rabbit Polyclonal Antibody

Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-PAX6 antibody (orb612166), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

PAX6 Rabbit Polyclonal Antibody

Sample: Cerebellum (Mouse) Lysate at 40 ug, Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-PAX6 (orb612166) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50/33 kD, Observed band size: 50 kD.

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