PARP14 Rabbit Polyclonal Antibody

• Catalog Number: orb312946
• Category: Antibodies
• Description: PARP14 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Sheep, Zebrafish
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PARP14 (1651-1750/1801aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 203 kDa
• Target: PARP14
• UniProt ID: Q460N5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ADP-ribosyltransferase diphtheria toxin-like 8; AR
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

X Yu, T Yang, D Wu, C Xu, Z Li, A Sun, S Gao, H Li, Z Fan, R Huang PARP14 inhibits microglial activation via NNT to alleviate depressive-like behaviors in mice Brain, Behavior, and Immunity, (2025)

PubMed:

Applications

WB

Reactivity

Mouse

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PARP14) Polyclonal Antibody, Unconjugated (orb312946) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.