PARP14 Rabbit Polyclonal Antibody

SKU: orb312946

Description

PARP14 Rabbit Polyclonal Antibody

Research Area

ADP-ribosylation, Cell Signaling, SARS /COVID-19

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Sheep, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PARP14 (1651-1750/1801aa)
• Target: PARP14
• Molecular Weight: 203 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ADP-ribosyltransferase diphtheria toxin-like 8; ARTD8; B aggressive lymphoma protein 2; BAL2; Collaborator of STAT6; KIAA1268; PAR14_HUMAN; PARP 14; PARP-14; PARP14; pART8; Poly(ADP ribose) polymerase family member 14; Poly [ADP-ribose] polymerase 14.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PARP14) Polyclonal Antibody, Unconjugated (orb312946) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

PARP14

UniProt

Q460N5

References

X Yu, T Yang, D Wu, C Xu, Z Li, A Sun, S Gao, H Li, Z Fan, R Huang PARP14 inhibits microglial activation via NNT to alleviate depressive-like behaviors in mice Brain, Behavior, and Immunity, (2025)

PubMed:

Applications

WB

Reactivity

Mouse