PARP1 Rabbit Polyclonal Antibody

• Catalog Number: orb13638
• Category: Antibodies
• Description: PARP1 Rabbit Polyclonal Antibody
• Target: PARP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human PARP (201-300/1014aa)
• UniProt ID: P09874
• RRID: AB_10931332
• MW: 111 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=0.2μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ADP ribosyltransferase(NAD+; poly(ADP ribose) poly
• Note: For research use only

Blank control (blue line): HL60 cells (blue). Primary Antibody (green line): Rabbit Anti-PARP antibody (orb13638), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: 293T. Primary Antibody (green line): Rabbit Anti-PARP1 antibody (orb13638), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: 293T (Human) Cell Lysate at 30 ug, Lane 2: HL60 (Human) Cell Lysate at 30 ug, Lane 3: K562 (Human) Cell Lysate at 30 ug, Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 5: U251 (Human) Cell Lysate at 30 ug, Lane 6: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-PARP (orb13638) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 115 kD.