Parkin/PRKN Antibody

• Catalog Number: orb234353
• Category: Antibodies
• Description: Parkin/PRKN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunocytochemistry , 0.5-1μg/ml, Mouse, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51641 MW
• UniProt ID: O60260
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: E3 ubiquitin-protein ligase parkin;Parkin;6.3.2.-;
• Note: For research use only
• Application notes: WB: The detection limit for Parkin is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC(P) analysis of Mouse Brain Tissue using Anti-Parkin Picoband antibody.

IHC(P) analysis of Rat Brain Tissue using Anti-Parkin Picoband antibody.

IHC(P) analysis of Human Intestinal Cancer Tissue using Anti-Parkin Picoband antibody.

Western blot analysis using Anti-Parkin Picoband antibody.Lane 1:U87 Cell;2:Mouse Brain Tissue.

IHC analysis of Parkin using anti-Parkin antibody.Parkin was detected in immunocytochemical section of NEURO-2α Cell.