PAR4 Rabbit Polyclonal Antibody

SKU: orb783031

Description

PAR4 Rabbit Polyclonal Antibody

Research Area

G Protein-Coupled Receptors, GPCR Signaling Pathway

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Proteinase-activated receptor 4 (301-385/385aa)
• Target: F2RL3
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

F2RL3; F2R Like Thrombin/Trypsin Receptor 3; Coagulation Factor II(Thrombin) Receptor-Like 3; Thrombin Receptor-Like 3; PAR-4; Coagulation Factor II Receptor-Like 3; Proteinase-Activated Receptor-4; Proteinase-Activated Receptor 4; Protease-Activated Receptor-4; PAR4_HUMAN;

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Lung tissue lysates, Lane 4: Rat Thyroid gland tissue lysates, Lane 5: Rat Testis tissue lysates, Lane 6: Human K562 cell lysates, Lane 7: Human THP-1 cell lysates, Lane 8: Human Hela cell lysates, Lane 9: Human Huvec cell lysates, Primary: Anti-Proteinase-activated receptor 4/PAR4 (orb783031) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41/38 kD.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PAR4 Polyclonal Antibody, Unconjugated (orb783031) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

F2RL3

UniProt

Q96RI0