F2R Antibody

SKU: orb1246406

Description

F2R Antibody

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2). Recommended concentration: 0.3-1µg/mL. Primary incubation 1 hour at room temperature.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-TRARRPESKATNAT
• Target: F2R
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

F2R, coagulation factor II (thrombin) receptor, CF2R, HTR, PAR1, TR, coagulation factor II receptor, protease-activated receptor 1, thrombin receptor

orb1246406 (0.3 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246406 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

Quick Database Links

Gene Symbol

F2R

UniProt

P25116

RefSeq (Protein):NP_001983.2