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Pan Cytokeratin Antibody Cocktail

Catalog Number: orb2636595

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb2636595
CategoryAntibodies
DescriptionAnti-cytokeratin clone rPCK/6750 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, rPCK/6750 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, rPCK/6750 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
ClonalityRecombinant
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
PurityProtein A/G affinity
ImmunogenCrude cytokeratin extract prepared from RT-4 and MCF-7 cells was used as the immunogen for the recombinant Pan-CK antibody.
Tested applicationsFACS, IF, IHC-P
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application notesOptimal dilution of the recombinant Pan-CK antibody should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberCocktail rPCK/6750
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis recombinant Pan-CK antibody is available for research use only.
NoteFor research use only
Pan Cytokeratin Antibody Cocktail

IHC staining of FFPE human prostate tissue with recombinant Pan-CK antibody (clone rPCK/6750). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Pan Cytokeratin Antibody Cocktail

SDS-PAGE analysis of purified, BSA-free recombinant Pan-CK antibody (Cocktail rPCK/6750) as confirmation of integrity and purity.

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    FACS,  IF,  IHC-P,  WB

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    FACS,  IF,  IHC-P,  WB

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