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Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

Catalog Number: orb2644183

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2644183
CategoryAntibodies
DescriptionAnti-cytokeratin clone PCK/3150 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/3150 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/3150 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG2a, kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/Preservatives0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenA crude cytokeratin extract prepared from RT-4 and MCF-7 cells was used as the immunogen for this Pan Cytokeratin antibody.
Tested applicationsFACS, IF, IHC-P
Dilution rangeFlow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application notesOptimal dilution of the antibody should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberPCK/3150
Formula0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis Pan Cytokeratin antibody is available for research use only.
NoteFor research use only
Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

Immunofluorescent staining of permeabilized human HeLa cells with Pan Cytokeratin antibody (clone PCK/3150, green) and Reddot nuclear stain (red).

Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

Flow cytometry testing of permeabilized human HeLa cells with Pan Cytokeratin antibody (clone PCK/3150); Red=isotype control, Blue= Pan Cytokeratin antibody.

Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

IHC staining of FFPE human prostate with Pan Cytokeratin antibody (clone PCK/3150). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

IHC staining of FFPE human prostate with Pan Cytokeratin antibody (clone PCK/3150). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

SDS-PAGE analysis of purified, BSA-free Pan Cytokeratin antibody (clone PCK/3150) as confirmation of integrity and purity.

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