PAK4 Antibody

SKU: orb1239294

Description

PAK4 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  PAK4 antibody can be used for the detection of PAK4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: PAK4 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4.The immunogen is located within amino acids 210 - 260 of PAK4.
• Target: PAK4
• Molecular Weight: Predicted: 65 kDa Observed: 70 kDa
• Purification: PAK4 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PAK4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PAK4 Antibody: KIAA1142, p21-activated kinase 4, PAK-4

Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 µg/mL.

Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

PAK4

UniProt

O96013

RefSeq (Protein):NP_005875