PAI1 Rabbit Polyclonal Antibody

SKU: orb11226

Description

PAI1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PAI-1 (301-402/402aa)
• Target: SERPINE1
• Molecular Weight: 46 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PAI; PAI-1; PAI1; PLANH1; PAI1A; Pai1aa; Planh; RATPAI1A; PAI1_BOVIN; SERPINE1; Endothelial plasminogen activator inhibitor; Serpin E1; PAI1_HUMAN; PAI1_MOUSE; Mr1; PAI1_PIG; PAI1_RAT; serpin family E member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; plasminogen activator inhibitor, type I

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PAI1) polyclonal Antibody, Unconjugated (orb11226) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PAI1) polyclonal Antibody, Unconjugated (orb11226) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Rat Lung tissue lysates, Primary: Anti-PAI1 (orb11226) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 46 kDa.

Quick Database Links

Gene Symbol

SERPINE1

UniProt

P05121