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Item 1 of 2
Item 1 of 2
PAG3/ASAP2 Polyclonal Antibody
Catalog Number: orb1417817
| Catalog Number | orb1417817 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to PAG3. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
| Reactivity | Porcine, Rat |
| Conjugation | Unconjugated |
| UniProt ID | O43150 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Arf-GAP with SH3 domain, ANK repeat and PH domain- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Sample: Cerebrum (Mouse) Lysate at 40 ug. Primary: Anti- PAG3/ASAP2 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 112 kD. Observed band size: 110 kD.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PAG3 ASAP2) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
