PAG antibody

• Catalog Number: orb44470
• Category: Antibodies
• Description: Mouse monoclonal antibody to PAG
• Clonality: Monoclonal
• Clone Number: PAG-C1
• Tested applications: IHC-P, IP, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1. Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: PAG1
• Entrez: 94212
• UniProt ID: Q3U1F9
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: PAG antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1. Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG (MEM-255; cat. no. orb44227). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1, 2: immunoprecipitation with anti-PAG (PAG-C1); Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody; cat. no. orb44781).

Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in human appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.