PADI6 Rabbit Polyclonal Antibody

• Catalog Number: orb312631
• Category: Antibodies
• Description: PADI6 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Human
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PADI6 (101-200/694aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 77 kDa
• Target: PADI6
• UniProt ID: Q6TGC4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: hPADVI; PADI6; PADI6_HUMAN; peptidyl arginine deim
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Juepu Zhou 1, Ruolin Mao 1, Limin Gao 1, Meng Wang 1, Rui Long 1, Xiangfei Wang 1, Zhou Li 2, Lei Jin 3, Lixia Zhu Novel variants in PADI6 genes cause female infertility due to early embryo arrest J Assist Reprod Genet, (2024)

PubMed: 39644447

Applications

IF

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PADI6) Polyclonal Antibody, Unconjugated (orb312631) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PADI6) Polyclonal Antibody, Unconjugated (orb312631) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.