PACSIN2 Antibody

• Catalog Number: orb1262761
• Category: Antibodies
• Description: PACSIN2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the C-terminal region of human PACSIN2.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:100For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: PACSIN2
• UniProt ID: Q9UNF0
• NCBI: Q9UNF0
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Protein kinase C and casein kinase substrate in ne
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:100For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. stomach section using PACSIN2 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.stomach section using PACSIN2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Fluor 554 (red) conjugated Phalloidin (red).

Western blot analysis in Daudi cell line and mouse brain lysates (35 ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).

Western blot analysis in Daudi, HL-60 cell line and mouse brain, rat heart lysates (35 ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.