PACSIN2 Antibody (C-term)

• Catalog Number: orb1788460
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the C-terminal region of human PACSIN2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: 342-371 aa
• UniProt ID: Q9UNF0
• MW: 55739 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:100, WB: 1:1000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:100
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PACSIN2; Protein kinase C and casein kinase substr
• Note: For research use only
• NCBI: NP_001171899.1, NP_001171900.1, NP_009160.2

PACSIN2 Antibody (S357) western blot analysis in Daudi cell line and mouse brain lysates (35 ug/lane). This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).

Immunohistochemical analysis of paraffin-embedded H. stomach section using PACSIN2 Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.stomach section using PACSIN2 Antibody (C-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from rat heart tissue lysate and Daudi cell line (from left to right), using PACSIN2 Antibody (S357). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody (C-term). Diluted at 1: 100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).