P70 S6 kinase beta Rabbit Polyclonal Antibody

• Catalog Number: orb2779
• Category: Antibodies
• Description: P70 S6 kinase beta Rabbit Polyclonal Antibody
• Target: RPS6KB2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human RPS6KB2 (281-380/525aa)
• UniProt ID: Q9UBS0
• MW: 57 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1;
• Note: For research use only

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-p70 S6 kinase beta antibody (orb2779), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Thymus tissue lysates, Lane 3: Mouse NIH/3T3 cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human PANC-1 cell lysates, Lane 6: Human MCF-7 cell lysates, Lane 7: Human HeLa cell lysates, Primary: Anti-p70 S6 kinase beta (orb2779) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kDa, Observed band size: 55 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-p70 S6 kinase beta Polyclonal Antibody, Unconjugated (orb2779) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.