RPS6KB1 Antibody

SKU: orb420314

Description

p70 S6 Kinase antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000, IP: 5 µg/mg protein sample, WB: 1:1,000
• Reactivity: Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 70 kDa in size corresponding to p70S6K by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p70S6K protein.
• Target: RPS6KB1
• Purity: This affinity-purified antibody is directed against the human p70S6K protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p70S6K proteins from human, rat and mouse. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

p70S6Kb antibody, Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody, Ribosomal protein S6 kinase beta 2 antibody

Western blot using Biorbyt's Affinity Purified anti-p70S6K antibody shows detection of a predominant band corresponding to p70S6K in mouse brain extract (right lane) after immunoprecipitation. The left lane shows mock immunoprecipitation.

Quick Database Links

Gene Symbol

RPS6KB1

UniProt

P23443

RefSeq:54041234