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p70 S6 Kinase Antibody

SKU: orb420314

Description

p70 S6 Kinase antibody

Images & Validation

Tested ApplicationsELISA, IP, WB
Dilution rangeELISA: 1:5,000, IP: 5 µg/mg protein sample, WB: 1:1,000
ReactivityMouse
Application Notes
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 70 kDa in size corresponding to p70S6K by western blotting in the appropriate cell lysate or extract.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p70S6K protein.
PurityThis affinity-purified antibody is directed against the human p70S6K protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p70S6K proteins from human, rat and mouse. Reactivity against homologues from other sources is not known.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration1.0 mg/mL
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

p70S6Kb antibody, Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody, Ribosomal protein S6 kinase beta 2 antibody

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p70 S6 Kinase Antibody

Western blot using Biorbyt's Affinity Purified anti-p70S6K antibody shows detection of a predominant band corresponding to p70S6K in mouse brain extract (right lane) after immunoprecipitation. The left lane shows mock immunoprecipitation.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeq54041234

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

p70 S6 Kinase Antibody (orb420314)

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