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p70 S6 Kinase Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IP, WB |
|---|---|
| Dilution range | ELISA: 1:5,000, IP: 5 µg/mg protein sample, WB: 1:1,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p70S6K protein. |
| Purity | This affinity-purified antibody is directed against the human p70S6K protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p70S6K proteins from human, rat and mouse. Reactivity against homologues from other sources is not known. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
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Western blot using Biorbyt's Affinity Purified anti-p70S6K antibody shows detection of a predominant band corresponding to p70S6K in mouse brain extract (right lane) after immunoprecipitation. The left lane shows mock immunoprecipitation.
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Protocol Information
p70 S6 Kinase Antibody (orb420314)
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