p65 Antibody / NF-kB

• Catalog Number: orb1151613
• Category: Antibodies
• Description: Mouse monoclonal antibody to p65
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Recombinant full-length human RELA protein was used as the immunogen for the p65 NF-kB antibody.
• UniProt ID: Q04206
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Application notes: Optimal dilution of the p65 NF-kB antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-RELA-1E3
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the p65 NF-kB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This p65 NF-kB antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of PFA-fixed human MCF-7 cells using p65 NF-kB antibody (green, clone PCRP-RELA-1E3) and phalloidin (red).

FACS staining of PFA-fixed human K562 cells with p65 NF-kB antibody (blue, clone PCRP-RELA-1E3) and isotype control (red).

FACS staining of PFA-fixed human HeLa cells with p65 NF-kB antibody (blue, clone PCRP-RELA-1E3) and isotype control (red).

FACS staining of PFA-fixed human U-87 cells with p65 NF-kB antibody (blue, clone PCRP-RELA-1E3), and isotype control (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using p65 NF-kB antibody (clone PCRP-RELA-1E3). These results demonstrate the foremost specificity of the PCRP-RELA-1E3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free p65 NF-kB antibody (PCRP-RELA-1E3) as confirmation of integrity and purity.