P53BP1 Rabbit Polyclonal Antibody

SKU: orb6593

Description

P53BP1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human p53BP1/53BP1 (501-600/1977aa)
• Target: TP53BP1
• Molecular Weight: 213 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

53 BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; TP53 BP1; TP53BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; TP53B_HUMAN; tumor protein 53-binding protein, 1.

Tissue/Cell: rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-p53BP1 Polyclonal Antibody, Unconjugated (orb6593) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

TP53BP1

UniProt

Q12888

Yang, Shanshan et al. Combination of RIZ1 Overexpression and Radiotherapy Contributes to Apoptosis and DNA Damage of HeLa and SiHa Cervical Cancer Cells Basic Clin Pharmacol Toxicol, 123, 137-146 (2018)

PubMed: 29575614