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Catalog Number | orb2315200 |
---|---|
Category | Antibodies |
Description | p53 (Tumor Suppressor Protein) Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | PCRP-TP53-1F7 |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Recombinant human full-length TP53 protein |
Concentration | Purified Ab WITHOUT BSA and Azide at 1.0mg/ml |
Dilution range | Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Conjugation | Unconjugated |
MW | 53kDa. |
Target | TP53 |
Entrez | 7157 |
UniProt ID | P04637 |
Expression System | HEK293 cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53 Read more... |
Note | For research use only |
Application notes | Positive Control: HeLa cells. Colorectal or Ovarian Carcinoma |
Expiration Date | 12 months from date of receipt. |
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Equine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Equine | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P | |
Canine | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |