p53 antibody

• Catalog Number: orb668471
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p53
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IH, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human p53. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000, IHC-P: 1-50-200, IP: 1-20-100, ChIP: 1-20-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: TP53
• Entrez: 7157, 22059, 24842
• UniProt ID: P10361, P04637, P02340
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-P53 antibody, anti-Cellular tumor antigen p53
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of p53 expression in MCF7 (A), mouse spleen (B), rat kidney (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 53 kD)

Immunohistochemical analysis of p53 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.