p53 Antibody

• Catalog Number: orb1735095
• Category: Antibodies
• Description: p53 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DO-1
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Mouse IgG1
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200 – 1:300 Immunohistochemistry (Paraffin) 1:100
• Conjugation: Unconjugated
• Target: p53, monoclonal antibody [DO-1]
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-p53 monoclonal antibody [DO-1] at 1/500 dilution. Lysates at 30 μg per lane. NC membrane was then blocked for an hour before being incubated with orb1735095 overnight at 4°C.

HCT-116 cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1735095 Anti-p53 monoclonal antibody [DO-1] (green) at 1:200 and 4°C.

Immunohistochemical analysis of paraffin embedded Human tissue labeling p53 monoclonal antibody [DO-1] with orb1735095 at 1/100.