Cart summary

You have no items in your shopping cart.

    P4HA1 Antibody

    Catalog Number: orb1247152

    DispatchUsually dispatched within 5-10 working days
    $ 813.00
    Catalog Numberorb1247152
    CategoryAntibodies
    DescriptionP4HA1 Antibody
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    Predicted ReactivityCanine, Mouse, Rat
    ReactivityHuman
    ImmunogenThe immunogen for this antibody is: C-DFARKDEPDAFKE
    Concentration500 ug/mL
    Dilution rangePeptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1ug/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Spleen. Recommended concentration: 5ug/ml.
    Form/AppearanceLiquid
    ConjugationUnconjugated
    TargetP4HA1
    UniProt IDP13674
    NCBINP_000908.2, NP_001017962.1
    StorageAliquot and store at -20°C. Minimize freezing and thawing.
    Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
    Alternative namesP4HA1, procollagen-proline, 2-oxoglutarate 4-dioxy
    Read more...
    NoteFor research use only
    Application notesPeptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1ug/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Spleen. Recommended concentration: 5ug/ml.
    Expiration Date12 months from date of receipt.
    P4HA1 Antibody

    orb1247152 (5 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    P4HA1 Antibody

    orb1247152 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    • P4HA1 antibody [orb19434]

      ELISA,  IF,  IHC

      Bovine, Canine, Human

      Goat

      Polyclonal

      Unconjugated

      100 μg
    • P4HA1 antibody [orb50160]

      ELISA,  IHC,  WB

      Human, Rat

      Rabbit

      Polyclonal

      Unconjugated

      50 μg, 100 μg
    • P4HA1 antibody [orb411795]

      IF,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      200 μl, 100 μl, 50 μl
    • P4HA1 antibody [orb395399]

      ELISA,  IF,  IHC,  WB

      Human, Mouse, Rat

      Mouse

      Monoclonal

      Unconjugated

      100 μg
    • P4HA1 antibody [orb331162]

      WB

      Animal, Bovine, Canine, Human, Mouse, Porcine, Rat

      Canine, Equine, Human, Mouse, Porcine, Rat

      Rabbit

      Polyclonal

      Unconjugated

      100 μl
    Submit a review

    Filter by Rating

      • Star
      • Star
      • Star
      • Star
      • Star
      • 5 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 4 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 3 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 2 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 1 stars