p38 MAPK (phospho Thr180 / Tyr182) Antibody

• Catalog Number: orb1273517
• Category: Antibodies
• Description: p38 MAPK (phospho Thr180 / Tyr182) Antibody
• Target: Mapk14
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Purification: Affinity Purified
• Immunogen: p38 MAPK (Thr180/Tyr182) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr180/Tyr182 of p38.
• UniProt ID: P70618
• MW: 39
• Tested applications: WB
• Application notes: Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Immunoblotting (DB) and Western Blots (WB). Immunohistochemistry (IHC): not yet determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: P70618

Western blot of Hela cell lysates that had been treated with + /- UV (30 min.) showing phosphospecific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.