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p35 Control protein

SKU: orb345965

Description

p35 Control protein

Research Area

Protein Biochemistry

Images & Validation

Tested ApplicationsSDS-PAGE, WB
Application Notes
p35 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 69.5 kDa for p35-MBP, (27.1 kDa for p35 and 42.4 kDa for MBP) in size corresponding to p35 by Western blotting in the appropriate cell lysate or extract. p35 kDa protein has been tested in SDS-page.

Key Properties

SourceBorrelia burgdorferi
Expression SystemE. coli
Biological OriginBorrelia burgdorferi
TargetBB-H32, bba64
ConjugationUnconjugated
Purityp35 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified p35 and was estimated to be greater than 90% pure.

Storage & Handling

StorageStore vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1 mg/ml
Expiration Date6 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

bba64, Borrelia burgdorferi p35, control protein

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

100 μg
$ 780.00
DispatchUsually dispatched within 5-10 working days
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