p35 antibody

• Catalog Number: orb344671
• Category: Antibodies
• Description: p35 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.
• Concentration: 1.4 mg/mL
• Dilution range: ELISA: >1:5,000, WB: 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p35 protein. A BLAST analysis was used to suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence. Cross-reactivity with p35 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: O50687
• NCBI: NP_045522.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-p35 Antibody, bba64, Borrelia burgdorf
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 27.1 kDa in size corresponding to Borrelia burgdorferi p35 protein by Western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Molecular weight markers. Lane 2: MBP-p35 fusion protein (arrow; expected MW: 69.5 kDa). Lane 3: MBP alone using p35 antibody