P2RX4 antibody

• Catalog Number: orb381935
• Category: Antibodies
• Description: Rabbit polyclonal antibody to P2RX4.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant full length protein of human P2X4
• Dilution range: WB: 1:500-2000, IF/ICC: 1:10-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: P2RX4
• Entrez: 5025
• UniProt ID: Q99571
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: P2X purinoceptor 4; P2X4; ATP receptor; Purinergic
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of P2X4 expression in SW480 (A), THP1 (B) whole cell lysates. (Predicted band size: 40; 43; 45 kD; Observed band size: 62 kD)

Immunofluorescent analysis of P2X4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.