P2RX4 antibody

• Catalog Number: orb100036
• Category: Antibodies
• Description: P2RX4 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Human
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human P2RX4 (238-338/388 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: P2RX4
• UniProt ID: Q99571
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: ATP gated cation channel protein; ATP receptor; p2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ronald Sluyter 1, Tahnee B-D McEwan 2, Reece A Sophocleous 2, Leanne Stokes 3 Methods for studying P2X4 receptor ion channels in immune cells J Immunol Methods, 526, 113626 (2024)

PubMed: 38311008

Applications

Activity Assay

Aminin, Dmitry et al. Glycosides from edible sea cucumbers stimulate macrophages via purinergic receptors Sci Rep, 6, 39683 (2016)

PubMed: 28004778

Applications

FC

Reactivity

Mouse

Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). P2RX4 antibody at 1:200 dilution with overnight incubation at 4 degrees

Paraffin-embedded mouse colon tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). P2RX4 antibody at 1:200 dilution with overnight incubation at 4 degrees