p27 (phospho Thr187) rabbit pAb

• Catalog Number: orb764293
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p27 (phospho-Thr187)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Thr187. AA range:149-198
• MW: 22kD
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CDKN1B antibody, anti-KIP1 antibody, anti-Cyc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-p27 (T187) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p27 Kip1 (Phospho-Thr187) Antibody.

Western blot analysis of lysates from HeLa cells treated with EGF or IFN-α, using p27 Kip1 (Phospho-Thr187) Antibody. The lane on the left is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).