p16INK4a Antibody

• Catalog Number: orb1806435
• Category: Antibodies
• Description: p16INK4a Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human
• Immunogen: Human p16 INK4A
• Concentration: 100 µg/ml
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: p16INK4a
• UniProt ID: Q8N726
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: P16-INK4A; multiple tumor suppressor 1; P14ARF; P1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human p16INK4a by western blot. Samples: Whole cell lysate (50 µg) from SK-MEL-28, HeLa, OVCAR-3, and OVCAR-8 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody [BC42] (orb1806435) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human p16INK4a by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Mouse anti-p16INK4a recombinant monoclonal antibody [BC42] (orb1806435). Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human p16INK4a (shaded) in HeLa cells by flow cytometry. Antibody: Mouse anti-p16INK4a recombinant monoclonal antibody [BC42] (orb1806435) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-mouse IgG.

Detection of human p16INK4a by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Mouse anti-p16INK4a recombinant monoclonal antibody [BC42] (orb1806435). Secondary: HRP-conjugated goat anti-mouse IgG.