P-cadherin Rabbit Polyclonal Antibody

SKU: orb11243

Description

P-cadherin Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Guinea pig, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human P-cadherin (625-725/829aa)
• Target: CDH3
• Molecular Weight: 80 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

placental; Cadherin-3; P cadherin; Cadp; Placental cadherin; P-cadherin; AI385538; Pca; Cadherin 3 precursor; Cadherin 3 type 1; Calcium dependent adhesion protein placental; Cadherin; CDH3; CDHP; 7B4 antigen; Cadherin-1; Cadherin-2; Cadherin-4; Cadherin-5; CAM 120/80; CD144; CD324; CD325; Cdh4; CDH5; CDHE; CDHN; CADH3_HUMAN; E-cadherin; Epithelial cadherin; N-cadherin; NCAD; Neural cadherin; P-cadherin; UVO; Uvomorulin; Vascular endothelial cadherin; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; PCAD; Placental cadherin.

Overlay histogram showing Mouse SP2/0 (red line). The cells were fixed with 1% paraformaldehyde (10 min). The cells were then incubated with the antibody (2ug/1x106cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of > 5000 events was performed.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-P-cadherin (orb11243) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 80 kD, Observed band size: 90 kD.

Tissue/Cell: U251 cells, 4% Paraformaldehyde-fixed, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-P-cadherin Polyclonal Antibody, Unconjugated (orb11243) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

CDH3

UniProt

P22223