P-cadherin Rabbit Polyclonal Antibody

• Catalog Number: orb11243
• Category: Antibodies
• Description: P-cadherin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Guinea pig, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human P-cadherin (625-725/829aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 80 kDa
• Target: CDH3
• UniProt ID: P22223
• RRID: AB_10752488
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: placental; Cadherin-3; P cadherin; Cadp; Placental
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing Mouse SP2/0 (red line). The cells were fixed with 1% paraformaldehyde (10 min). The cells were then incubated with the antibody (2ug/1x106cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of > 5000 events was performed.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-P-cadherin (orb11243) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 80 kD, Observed band size: 90 kD.

Tissue/Cell: U251 cells, 4% Paraformaldehyde-fixed, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-P-cadherin Polyclonal Antibody, Unconjugated (orb11243) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.