Oxphos Cell Survival Assay Kit

• Catalog Number: orb342312
• Category: Assays and Kits
• Description: Oxphos Cell Survival Assay Kit
• Conjugation: Unconjugated
• Storage: Store kit at 2-8° C prior to opening. See kit insert for complete instructions.
• Alternative names: Glutathione recycling, glutathione function, and g
• Note: For research use only
• Application notes: Oxphos gives a linear response for mammalian cells (0; 100,000; 200,000; 400,000; 600,000; and 800,000) plated in 1mL growth medium in a six well plate with up to 15% fetal bovine serum and measured 20 hours after plating. It also gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 µL) suspended in a total volume of 200 µL saline in a microfuge tube and incubated with 10 µL of reagent 6 for 2hrs. Oxphos contains enough reagents for 100 assays using a 6-well plate.
• Expiration Date: Please enquire.

OxPhos™ Cell Survival Assay for human colon cancer cells in a 6 well plate. This assay gives a linear response for mammalian cells (0; 100000; 200000; 400000; 600000; and 800000) plated in 1 mL growth medium in a six well plate with up to 15% fetal bovine serum and measured 20 hours after plating. The background (BG) O.D. is between 0.231-0.235 that is subtracted from the data for each of the cell density. A mean r2 values of 0.97 demonstrate the dynamic range up to 1 million cells. y = 2.376x + 0.138 r2 = 0.964. y = 2.774x + 0.158 r2 = 0.976. y = 2.819x + 0.163 r2 = 0.976. y = 2.814x + 0.164 r2 = 0.973. y = 2.736x + 0.199 r2 = 0.965.

OxPhos™ gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 µl) suspended in a total volume of 200 µl saline in a microfuge tube and incubated with 10 µl of reagent 6 for 2hrs. OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood.

OxPhos™ gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 µl) suspended in a total volume of 200 µl saline in a microfuge tube and incubated with 10 µl of reagent 6 for 2hrs. OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood.

OxPhos™ gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 µl) suspended in a total volume of 200 µl saline in a microfuge tube and incubated with 10 µl of reagent 6 for 2hrs. OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood.

Schematic representation of the various pathways involved in the cellular interactions of HEDS. HEDS reacts spontaneously with glutathione (GSH) or in a reaction catalyzed by glutathione-S-transferase (GST) to produce mixed disulfide (MESSG) of GSH and mercaptoethanol (ME) (Pathway 1). The mixed disulfide MESSG reacts with GSH and produces ME and GSSG by the catalytic action of glutaredoxin (GRX) (Pathway 2). The glutathione disulfide GSSG reacts with NADPH and produces GSH by the catalytic action of glutathione reductase (GRase) (Pathway 3). The conversion of GSSG to GSH i.e. GSH recycling requires NADPH recycling (NADP+ NADPH) by oxidative pentose phosphate cycle (OPPC) (Pathway 4)