Ox-LDL Rabbit Polyclonal Antibody

• Catalog Number: orb10973
• Category: Antibodies
• Description: Ox-LDL Rabbit Polyclonal Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Full length protein from human plasma
• RRID: AB_10748068
• MW: 31 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ox-LDL: LDL(Copper oxidized); Cu2SO4 oxidized low
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Stephan Zeibiga, Manuela Büttcherb Silvia Goebela, Jessica Paulia, Annique Hungera, Martin Ungerera, Meinrad Gawazb, Götz Müncha The Scavenger Receptor CD68 Regulates Platelet Mediated Oxidized Low-Density Lipoprotein (oxLDL) eposition in Atherosclerotic Vessels at an Early Stage of Atherosclerosis in LDLR-/-/ApoBec-/- Mice Cell Physiol Biochem, 52, 681-695 (2019)

PubMed: 30921507

Applications

ELISA

Reactivity

Chinese Hamster

Wern Yew Ding, Majd B Protty, Ian G Davies, Gregory Y H Lip Relationship between lipoproteins, thrombosis, and atrial fibrillation Cardiovasc Res, 118, 716–731 (2022)

PubMed: 33483737

Applications

IF

Reactivity

Human

Wei Jiang 1, Chun Gan 1, Xindi Zhou 1, Qing Yang 1, Dan Chen 1, Han Xiao 1, Lujun Dai 2, Yaxi Chen 3, Mo Wang 1, Haiping Yang 4, Qiu Li 5 Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease Cardiovasc Diabetol, 22, 293 (2023)

PubMed: 37891556

Applications

IF

Reactivity

Human

Uchida, Yasumi et al. Pericoronary Adipose Tissue as Storage and Supply Site for Oxidized Low-Density Lipoprotein in Human Coronary Plaques PLoS One, 11, e0150862 (2016)

PubMed: 27010927

Applications

IHC

Reactivity

Human

Uchida, Yasumi et al. Human pericoronary adipose tissue as storage and possible supply site for oxidized low-density lipoprotein and high-density lipoprotein in coronary artery J Cardiol, 69, 236-244 (2017)

PubMed: 27209423

Applications

IHC

Reactivity

Human

Blank control: A431. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (orb10973) Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3 µg/Test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (orb10973), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (orb10973) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.