OTUD1 Rabbit Polyclonal Antibody

• Catalog Number: orb185712
• Category: Antibodies
• Description: OTUD1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human OTUD1 (141-240/481aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 51 kDa
• Target: OTUD1
• UniProt ID: Q5VV17
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: DUBA-7; DUBA7; OTDC1; OTU domain containing 1; OTU
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Weiwei Zhu # 1 2, Qianhui Zhang # 1, Leiming Jin # 1, Shuaijie Lou 1, Jiaxi Ye 1, Yaqian Cui 1, Yongqiang Xiong 2, Mengsha Lin 1, Guang Liang 3 4 5, Wu Luo 6 7, Zaishou Zhuang OTUD1 Deficiency Alleviates LPS-Induced Acute Lung Injury in Mice by Reducing Inflammatory Response Inflammation, (2024)

PubMed: 39037666

Meng-Yang Wang # 1 2 3, Tian-Xiang Yu # 1 2, Qin-Yan Wang 1 2, Xue Han 1 2, Xiang Hu 4, Shi-Ju Ye 2, Xiao-Hong Long 2, Yi Wang 2, Hong Zhu 4, Wu Luo 5, Guang Liang OTUD1 promotes hypertensive kidney fibrosis and injury by deubiquitinating CDK9 in renal epithelial cells Acta Pharmacol Sin, (2024)

PubMed: 38110583

Reactivity

Mouse

Qinyan Wang 1, Shiqi Liang 1, Jinfu Qian 2, Jiachen Xu 2, Qingsong Zheng 3, Mengyang Wang 4, Xiaochen Guo 3, Julian Min 3, Gaojun Wu 2, Zaishou Zhuang 3, Wu Luo 5, Guang Liang OTUD1 promotes isoprenaline- and myocardial infarction-induced heart failure by targeting PDE5A in cardiomyocytes Biochim Biophys Acta Mol Basis Dis, (2024)

PubMed: 38185350

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (orb185712) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (orb185712) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (orb185712) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human A431 cell lysates, Primary: Anti-OTUD1 (orb185712) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kDa, Observed band size: 60 kDa.