Osteocalcin Rabbit Polyclonal Antibody

• Catalog Number: orb1266
• Category: Antibodies
• Description: Osteocalcin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Osteocalcin (21-100/100aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 11 kDa
• Target: BGLAP
• UniProt ID: P02818
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Osteocalcin Propeptide; gamma-carboxyglutamic acid
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Fábio Vieira de Miranda-Filho 1, Stéfany Barbosa 1, Olavo Alcalde Panigali 1, Mirela Caroline Silva 1, Monique Gonçalves da Costa 1, Franciele da Silva Flores 1, Edilson Ervolino 2, Letícia Helena Theodoro 1, Osvaldo Magro-Filho 1, Leonardo Perez Faverani 1 Effect of local and systemic administration of atorvastatin for improving bone healing on critical defects Braz Dent J ., (2024)

PubMed: 39476113

Applications

IHC

Reactivity

Rat

citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium clei.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei stainin.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human MG63 cell lysates, Lane 3: Human A549 cell lysates, Lane 4: Human U-2 OS cell lysates, Primary: Anti-Osteocalcin (orb1266) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 11 kDa.